Background: The importance of analytical chemistry in occupational toxicology is vital; therefore, some methods of extraction and analysis have been defined for this purpose. In this research, solidified floating organic drop microextraction (SFODME) technique was selected and evaluated. This procedure was used for the first time to extraction trace amounts of iron and zinc cations in urine samples.Methods: This experimental study was designed to extract iron and zinc from urine samples using SFODME technique. This study consisted of several stages including stock preparation for analysis, SFODME, and flame atomic absorption spectrometry. In addition, the optimum extraction conditions, efficiency, and repeatability of results were investigated.Findings: Results showed that optimum ligand volume was 2 ml and under optimum conditions, the relative standard deviation in urine spiked samples of iron and zinc ions was 2.6% and 3.4%, respectively, and recovery was 97.4% and 96.6% for these two ions, respectively. Ligand volume, volume of solvent, and extraction temperature had a significant impact on the extraction of trace amount of iron and zinc from urine samples.Conclusion: This research showed that SFODME technique has advantages in determination of trace amounts of iron and zinc in urine samples. Decrease in sample volume, extraction time, and exposure of the operator to toxic substances are the main advantages of this technique.

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