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Background: Frozen dough technology makes it possible to access fresh bread with a minimum need for equipment and with minimal skilled personnel at any time. In this study the impact of freezing rate and degree of pre-fermentation on quality factors of Sangak frozen dough and its bread was assessed. Methods: The pre-fermentation was between 0 and 120 min (full proving time), for every 30 min, and the corresponding secondly (or final) fermentation was between 120 and 0 min, respectively, to obtain total fermentation time of 120 min. The dough was formed in a flat shape with 3, 6 or 9 mm thickness and prefermented. The prefermented doughs was frozen under -25 °C. It was followed by 1-day storage at -18 °C and then a second fermentation and then to finish the fermented dough was baked. Sangak frozen dough quality was assessed by measuring yeast survival after thawing. Characteristics of frozen dough bread were evaluated by measuring density after baking. The statistical analysis was performed in factorial test based on completely randomized design (p < 0.05). Findings: Results showed that viability of yeast increased by decreasing dough thickness, so that at 3 mm the most viability was observed. The maximum yeast viability was observed at short pre-fermentation (30 min). The lowest bread density was obtained at 3 mm thickness and 30 min pre-fermentation. Conclusion: Freezing had an adverse effect on quality factors of frozen dough and its bread. Short pre-fermentation and lower thickness can obtain optimum conditions to produce Sangak frozen dough. Keyword: Bread density, Yeast viability, freezing rate, Sangak bread