Volume 8, Issue 6 (2-2013)
HSR 2013, 8(6): 1098-1106 |
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Sarvi Moghanlo V, Chorom M, Motamedy H, Pourzamani H, Falah M. شناسایی گونه باکتریهای تجزیه کننده هیدروکربنها در خاکهای آلوده به نفت خام اهواز. HSR 2013; 8 (6) :1098-1106
URL: http://hsr.mui.ac.ir/article-1-455-en.html
URL: http://hsr.mui.ac.ir/article-1-455-en.html
1- PhD Candidate, Department of Soil Engineering, School of Agriculture, University of Lorestan, Khoramabad, Iran
2- Associate Professor, Department of Chemistry and Soil Pollution, School of Agriculture, Shahid Chamran University, Ahwaz, Iran
3- Assistant Professors, Department of Microbiology, School of Science, Shahid Chamran University, Ahwaz, Iran
4- PhD Candidate, Student Research Committee, Environment Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
5- MSc, Department of Environmental Management, Islamic Azad University, Sciences and Research Branch, Ahwaz, Iran
2- Associate Professor, Department of Chemistry and Soil Pollution, School of Agriculture, Shahid Chamran University, Ahwaz, Iran
3- Assistant Professors, Department of Microbiology, School of Science, Shahid Chamran University, Ahwaz, Iran
4- PhD Candidate, Student Research Committee, Environment Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
5- MSc, Department of Environmental Management, Islamic Azad University, Sciences and Research Branch, Ahwaz, Iran
Abstract: (757 Views)
Background: Long-term extraction and production of various oil derivatives causes contamination of soil adjacent to production and refining area. The main important issue is the ecological hazard of these pollutants. The main aim of the study was to identify and separate native bacteria that are responsible for degradation of oil contaminated fields. Methods: We prepared a compound sample of contaminated soil in the vicinity of oil wells drilled in Marun oil field of Ahwaz, Iran. Moreover, an uncontaminated sample was picked from the same area and deliberately contaminated with crude oil in a 5 wt% rate. The number of oil degrading bacteria was counted by MPN (Most probable number ) method. Then, the bacteria was cultured and isolated in a rich agar medium and late discrimination test was done by gram staining and biochemical tests at the level of family or genus. Findings: The number of bacteria in naturally contaminated and artificially soil was 6.8 × 105 and 3.5 × 107, respectively. The majority of bacteria responsible for oil degradation were Staphylococcus, Acinetobacter and Flavobacterium genus in naturally contaminated soil; however, Acinetobacter, Pseudomonas and Moraxella genus were the main bacteria in artificially-contaminated soil samples. Conclusion: It is recommended to be used for bioremediation of native organisms and bio-power and robust.
Type of Study: Research |
Subject:
education health and promotion
Received: 2020/07/16 | Accepted: 2013/02/15 | Published: 2013/02/15
Received: 2020/07/16 | Accepted: 2013/02/15 | Published: 2013/02/15
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