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Background: Disposal of pharmaceutical compounds to the environment as an emerging pollutants cause significantly concerns and it is necessary to use the new methods of sewage treatment for removal of these compounds. The aim of this study was to feasibility using of O3/H2O2 process on diclofenac inhibitory impact on the specific methanogenic activity of anaerobic biomass.Methods: Twelve anaerobic digestions test in the batch model were carried out before and after using an O3/H2O2 process at the 500 ml reactors with 30% anaerobic biomass and 70% substrate to survey the inhibitory impact of diclofenac on the specific methanogenic activity of anaerobic biomass. The replacement liquid method was used. Duration of each test range was 8-16 days.Findings: Cumulative methane production in concentrations of 25, 50, 100, 175, 250 and 350 mg/l of diclofenac were 764.24, 845.42, 82, 102.09, 25.42 and 1.64 ml, respectively. These productions were 8.61, 26.65, 4.1 and 4.1 ml in concentrations of 25, 100, 250 and 350 mg/l, respectively, after 180 min pretreatment with O3/H2O2 process. It was demonstrated that methane production was reduced in all concentrations after ozonation process.Conclusion: It is concluded that the high concentration of diclofenac has inhibitory effects on the anaerobic digesters and after ozonation process pretreatment on diclofenac, the intermediates compounds can be produced which probably had very diverse effects on the anaerobic bacteria in digesters. They had also a lower biodegradability.Key Words: Anaerobic Digestion, Diclofenac, Specific Methanogenic Activity (SMA), Advanced Oxidation Process

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