1- MS, Department of Mechanical Engineering, Jami Institute of Technology, Isfahan, Iran
2- Faculty Member, Department of Civil & Environmental Engineering, Jami Institute of Technology, Isfahan, Iran
Abstract: (1853 Views)
Background: Control the microbial pollution in public places especially in hospitals is the best way to prevent diseases. Searching for some microorganisms such as Klebsiella, E-coli, stafilcocs and Hetrotrophic bacteria and 71 strains of enterobacteriaceae family in the air and some equipment of hospital as the indexes of microbial diversity was the main purpose of this study. Also investigating the possibility of dispersing microorganisms through air condition system was another aim of this research. Methods: The microbial studies in this research were divided in two groups: assessing the microbial quality of the air and the microbial quality of different surfaces in hospital. The polluted air was passed through a Milipore filter using a vacuum pump. The microorganisms existed in the air were collected on the filter media. These collected microorganisms were identified by the standard methods. Several objects in the hospital were examined to find possible existence microorganisms. For this purpose a cotton swap was employed to pick up microbial samples from the objects. Then microorganisms in these samples were isolated, cultivated and identified by the standard methods. In this study plate culture method was used to count heterotrophic microorganisms. Also Klebsiella and E-coli were identified by multi tube culture. Findings: After performing several tests it was revealed that the hospital air was free of e-coli. On the other hand based on the findings of this study it was concluded that existed microorganisms in the air inside the hospital included staphylococcus schleiferi, staphylococcus hyicus and klebsiella pneumoniae. Also counting heterotrophic bacteria showed that they were numerous in the inside air. Conclusion: Based on the microbial tests performed before and after the filtration of air conditioning system it was found that this filter was not efficient to significantly remove the microbial pollution from the air. The examination of the areas such as doors, walls etc. showed that the mentioned microorganisms existed in the hospital.
Type of Study:
Research |
Subject:
education health and promotion Received: 2020/07/16 | Accepted: 2011/03/15 | Published: 2011/03/15